Detection and quantification of
microbes in natural environments
Rome, 18-21 May 2010. SItEMICRO organizes the second practical/theoretical course on the main techniques applied in microbial ecology. The microbial communities of different natural environments will be studied through an applicative approach. The sampling survey, the experimental design, the application of different molecular methods, and the phylogenetic tree construction will constitute the scientific topics of the course.
Participation is limited to 20 applicants. Excellent master students, PhD students, and young researchers interested in microbial ecology are invited to apply.
Practical and theoretical lessons will be held by experienced international researchers in the field. The course will be hosted at the brand-new laboratories of the Water Research Institute (Istituto di Ricerca Sulle Acque; IRSA-CNR, Monterotondo, Roma).
Applied techniques
1) Denaturing Gradient Gel Electrophoresis (DGGE) to profile the microbial diversity.
2) Fluorescence In Situ Hybridization (FISH) to quantify the main phyplogenetic bacterial groups.
3) Probe and phylogenetic tree design by applying the software ARB.
4) Introduction to quantitativePCR (q-PCR) in microbial ecology.
DEADLINES
05 March 2010 - Send a short CV (max 1 page) and a brief motivation letter (max 200 words) to
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. 09 March 2010- 20 successful applicants will be notified, according to their CV and scientific motivation. 23 March 2010 - Payment of the registration fee (220 €). The details for the bank transfer will be communicated. 10 May 2010- Submission of the contribution for personal presentation (.ppt file) to
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. 18 May 2010- Beginning of the 2nd SItEMICRO Course
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