T-RFLP protocol Terminal restriction fragment length polymorphism (T-RFLP) allows the fingerprinting of a community by analyzing the polymorphism of a certain gene. It is a high-throughput, reproducible method that allows the semi-quantitative analysis of the diversity of a particular gene in a community.
CMEIAS CMEIAS consists of several custom plug-ins for UTHSCSA ImageTool, a free image analysis software operating on a personal computer in a Windows NT or higher environment. The CMEIAS object classifier automatically categorizes each cell in the image into one of 11 main microbial morphotypes, including cocci, spirals, curved rods, U-shaped rods, regular straight rods, unbranched filaments, ellipsoids, clubs, rods with extended prostheca, rudimentary branched rods, and branched filaments.
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Several protocols from different labs
FISH, CARD-FISH, CLONE-FISH
Here, we provide a number of general FISH (Fluorescence In-Situ hybrydization) protocols which should serve as a basis for a successful hybridization. A troubleshooting for FISH in provided as well as a number of suggested probes.
BIOWWW
Molecular biology troubleshooting forum
MolecularLab
Forum sulle tecniche di biologia molecolare in italiano. Occorre una registrazione.
***RDP
The Ribosomal Database Project (RDP) provides ribosome related data and services, including online data analysis and aligned and annotated Bacterial 16SrRNA sequences.
***The Ribosomal Internal Spacer Sequence Collection (RISSC)
This is a database of ribosomal 16S-23S spacer sequences intended mainly for molecular biology studies in typing, phylogeny and population genetics.
Progetto Biocode Nuovo portale italiano di bioinformatica con materiale da scaricare e nuove utili informazioni nel campo della bioinformatica.
***The RNA World Website
This web resource lists Internet links on RNA related topics.
GOLD
Genome OnLine Database. A World Wide Web resource for comprehensive access to information regarding complete and ongoing genome projects, as well as metagenomes and metadata, around the world.
CAMERA A Community Cyberinfrastructure for Advanced Marine Microbial Ecology Research and Analysis. Portale per l'analisi di METAGENOMI da campioni marini.
MEGX:
Marine Ecological Genomix: Portale per l'analisi di METAGENOMI da campioni marini. Campioni georeferenziati con relativi dati ecologici al momento del campionamento.